Intego PV

Pestivirus Detection Kit

PCR kit for qualitative in vitro diagnosis of pestiviruses

RT-PCR assay for accurate and reliable detection of BVDV and other pestiviruses in various in situ biologicals including cell cultures.

Test Principle

Intego®PV employs a Reverse-Transcription Polymerase Chain Reaction (RT-PCR), thereby providing the highest sensitivity for the detection of pestivirus contamination in cell cultures and other cell culture derived biologicals. The test utilizes pan-pestivirus primers selected from the 5'untranslated region (5'-UTR) of the pestivirus genome.
Only one protocol is needed for pestivirus testing, which can be completed within 4 hours. Intego®PV also provides an internal control for the PCR to assure successfully executed reactions.

Background

The genus pestivirus comprises four approved species: Bovine Viral Diarrhea Viru s 1 (BVDV-1), BVDV-2, Classical Swine Fever Virus (CSFV), and Border Disease Virus (BDV). Studies have shown that BVDV contamination reaches levels of 20-30% among raw fetal calf serum (FCS), and up to 49% of commercially available FCS. Additionally, vaccines produced on contaminated cells may in turn be contaminated, leading to sero-conversion or disease in the vaccine.

Last modified: 2008-05-14 16:46:46

Benefits

• Specific - Pan-pestivirus primers of the 5´-UTR of the pestivirus genome are highly conserved among all virus isolates. Intego®PV detects different pestivirus species: Bovine viral diarrhea virus 1 (BVDV-1), Bovine viral diarrhea virus 2 (BVDV-2), Classical swine fever virus (CSFV), Border disease virus (BDV).
• Reliable - The kit contains positive control DNA, and an internal control confirming both reverse transcription and PCR
• Rapid - Results are obtained within 4 hours. Hand-on time less than 45 minutes
• Convenient - The reagents are pre-aliquoted for 25 reactions for convenient periodic or routine testing
• Ease of use - One easy-to-follow, step-by-step protocol detects different pestivirus contaminants. Samples are easily prepared by RNA extraction

Last modified: 2008-05-14 16:46:46