Hybridase™ Thermostable RNase H

Supplier: EPICENTRE® Biotechnologies

Hybridase™ Thermostable RNase H

Catalogue number

Pack size

H39100

100U

H39500

500U

Epicentre's® patented* Hybridase™ Thermostable RNase H specifically degrades only RNA in an DNA:RNA hybrid, without affecting DNA or unhybridised RNA. In contrast to E. coli RNase H, which is rapidly inactivated at 55°C, Hybridase RNase H is active at high temperatures. It has optimal activity above 65°C and can be used at temperatures up to 95°C. The thermostability of the enzyme permits it to be used at temperatures that give the highest hybridisation stringency for specific DNA:RNA heteroduplexes, maximising sensitivity and selectivity while minimising background due to nonspecific hybridisation.

Unit Definition:

One unit results in the acid-solubilisation of 1nmole of 3H-polyadenylic acid in the presence of an equimolar concentration of polythymidylic acid in 20 minutes at 45°C in 50mM Tris-HCl, pH 7.5, 100mM NaCl, and 10mM MgCl2.

Note: The unit assay is performed at 45°C because this is optimal for the Tm of poly(dT):poly(A). The optimal temperature for many applications may be considerably higher.

Storage Buffer:

50% glycerol containing 50mMTris-HCl, pH 7.5, 0.1M NaCl, 1.0 mM DTT, 0.1mM EDTA, and 0.1% Triton® X-100.

Quality Control:

Hybridase Thermostable RNase H is tested for RNA degradation in an RNA:DNA hybrid and for the absence of detectable DNA exo- or endonuclease, and non-RNase H RNase activities.

Epicentre®'s new AmpliCap-Max™ T7 and AmpliCap-Max™ T3 High Yield Message Maker Kits are specially formulated to produce the highest yield of 5'-capped RNA (m⁷G[5']ppp[5']G cap analog) from an in vitro transcription reaction in the shortest reaction time.

The new AmpliCap-Max™ T7 and AmpliCap-Max™ T3 High Yield Message Maker Kits feature:

Protocols for: FailSafe™ PCR System

(catalogue number FS99060 / FS99100 / FS99250 / FS9901K / FSE51100 / FSE5101K / FSP995A→L)

Please note: all protocols off site are the responsibility of the products supplier

References

Hybridase™ Thermostable RNase H

Supplier: EPICENTRE® Biotechnologies

Hybridase™ Thermostable RNase H

Unit Definition:

One unit results in the acid-solubilisation of 1nmole of 3H-polyadenylic acid in the presence of an equimolar concentration of polythymidylic acid in 20 minutes at 45°C in 50mM Tris-HCl, pH 7.5, 100mM NaCl, and 10mM MgCl2.

Storage Buffer:

50% glycerol containing 50mMTris-HCl, pH 7.5, 0.1M NaCl, 1.0 mM DTT, 0.1mM EDTA, and 0.1% Triton® X-100.

Quality Control:

Hybridase Thermostable RNase H is tested for RNA degradation in an RNA:DNA hybrid and for the absence of detectable DNA exo- or endonuclease, and non-RNase H RNase activities.

Epicentre®'s new AmpliCap-Max™ T7 and AmpliCap-Max™ T3 High Yield Message Maker Kits are specially formulated to produce the highest yield of 5'-capped RNA (m7G[5']ppp[5']G cap analog) from an in vitro transcription reaction in the shortest reaction time.

The new AmpliCap-Max™ T7 and AmpliCap-Max™ T3 High Yield Message Maker Kits feature:

Protocols for: FailSafe™ PCR System

(catalogue number FS99060 / FS99100 / FS99250 / FS9901K / FSE51100 / FSE5101K / FSP995A→L)

Please note: all protocols off site are the responsibility of the products supplier

References

Epicentre®'s new AmpliCap-Max™ T7 and AmpliCap-Max™ T3 High Yield Message Maker Kits are specially formulated to produce the highest yield of 5'-capped RNA (m⁷G[5']ppp[5']G cap analog) from an in vitro transcription reaction in the shortest reaction time.