FosmidMAX™ DNA Purification Kit

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Last modified: 2008-07-15 14:57:15

The FosmidMAX™ DNA Purification Kit was developed for easy, reliable isolation of high-quality fosmid DNA

The scalable protocol is based on a modified alkaline-lysis procedure that typically yields 0.6 to 25mg of fosmid DNA from 1.5 to 100ml of a single-copy fosmid culture. Up to 4mg of fosmid DNA can be isolated from 1.5ml of an induced CopyControl fosmid clone.

The exceptionally pure fosmid DNA can be used for many applications including sequencing, fingerprinting, PCR and preparation of shotgun libraries.

Figure 1. The FosmidMAX™ Kit yields high-quality fosmid DNA that can be used directly for downstream applications like fingerprinting. Fosmid DNA isolated from 6 different fosmid clones (Lanes 1 to 6) was digested with Hind III and analyzed by agarose gel electrophoresis. Lane M, kb ladder.		Figure 2. Fosmid clones made with the CopyControl™ Fosmid Library Production Kit can be induced from single-copy up to 50 copies per cell to greatly increase DNA yield. DNA from an equal number of cells of uninduced (-) and induced (+) CopyControl fosmid clones was isolated using the FosmidMAX™ Kit. Equivalent volumes of fosmid DNA were digested with Hind III and analyzed by agarose gel electrophoresis. Lane M, kb ladder.

Last modified: 2008-05-14 16:46:46

Applications

• Extremely Versatile: ideal for isolating DNA from single-copy fosmid clones, induced CopyControl™ fosmid clones and cosmids

Benefits

• Superior yields
• Easy to follow protocols
• Convenient: eliminate the need for columns, resins or organic extractions
• Consistent Results: selective precipitation steps and the incorporation of RiboShredder™ RNase Blend effectively remove contaminants that degrade DNA and interfere with downstream applications

Last modified: 2008-05-14 16:46:46

Protocols for: FosmidMax™ DNA Purification Kit

(catalogue number FMAX046)

Please note: all protocols off site are the responsibility of the products supplier

Last modified: 2008-05-14 16:46:46