Contents > Protein Purification > Fluoro-Spin™ Protein Labelling kit

Catalogue number
Description
Features
Protocols

Fluoro-Spin™ Protein Labelling kit

Supplier: Princeton Separations

FluoroSpin 496 - Call for pricing details

Catalogue numberPack size
MK-F010110 reactions FAM
MK-F01155 reactions FAM

FluoroSpin 557 - Call for pricing details

Catalogue numberPack size
MK-T010210 reactions TAMRA
MK-T01185 reactions TAMRA

FluoroSpin 587 - Call for pricing details

Catalogue numberPack size
MK-R010310 reactions rox
MK-R01215 reactions ROX

For pricing on FluoroSpin™ please contact us directly
prices excluded VAT and shipping

Last modified: 2008-05-14 16:46:46

FluoroSpin™ Fluorescent Protein Labeling kits are designed for the labeling of proteins, enzymes or antibodies with molecular weights greater than 25 kD. Proteins are labeled using a reactive succinimdyl-ester (NHS) of FAM (Carboxy-Fluorescein), TAMRA (Carboxy -Tetra methylrhodamine) or ROX (Carboxy-X- Rhodamine). A covalent amide linkage forms between the activated dye and protein lysine groups. NHS esters provide superior stability compared to ITC (isothiocyanate) conjugates. The protein-dye conjugates have absorption and excitation maxima that are compatible with the excitation line of an Argon laser.

Up to 1.5 µg of protein can be labeled with the activated dyes. Each kit contains enough reactive dye for up to five labeling reactions. Centri-Sep™ spin columns are provided for removal of unbound dye from labeled protein conjugates. Quick start optimized protocols are provided for the labeling of 100 µg, 1 mg of IgG or up to 1.5 mg protein. Detailed instructions are provided for changing the number of dye labels linked to a protein. Kits are stable for six months in the dark at room temperature (15°C - 20°C).

Last modified: 2008-05-14 16:46:46

For pricing on FluoroSpin™ please contact us directly
prices excluded VAT and shipping

Last modified: 2008-05-14 16:46:46

Protocols for: Fluoro.Spin


A sample Fluoro.Spin Protocol

(catalogue number MK-F0101 / MK-F0115)


Please note: all protocols off site are the responsibility of the products supplier

Last modified: 2008-05-14 16:46:46

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