Fast-Link™ DNA Ligation Kits

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Last modified: 2008-07-15 14:57:15

The Fast-Link™ DNA Ligation Kits are specially formulated to provide the fastest high efficiency DNA ligations for routine and high throughput DNA cloning. Cohesive-end ligations can be performed in as little as 5 minutes at room temperature. In addition, desalting a Fast-Link™ ligation reaction prior to transformation of electrocompetent or chemically competent cells is not necessary.

Last modified: 2008-05-14 16:46:46

Applications

• TA cloning
• PCR blunt-end cloning
• Genomic DNA cloning and subcloning
• BAC library construction
• cDNA cloning
• Linker ligation

Benefits

• Cohesive end ligations in 5 minutes at room temperature(Figure 1)
• Blunt end ligations in 15 minutes at room temperature (Figure 2)
• Ligation of PCR product with A-overhangs in 1 hour at 16°C

Figure 1. Time course for cohesive-end ligation using the Fast-Link™ Kit. Lambda Hind III markers were ligated using 2 units of Fast-Link DNA Ligase (Lanes 3-7). Lane 1, kb ladder; Lane 2, no enzyme.

Figure 2. Time course for blunt-end ligation using the Fast-Link™ Kit. Pvu II digested pUC19 was ligated using 2 units of Fast-Link DNA Ligase (Lanes 2-7). Lanes 1 and 8, kb ladder.

Last modified: 2008-05-14 16:46:46

Protocols for: Fast-Link™ DNA Ligation Kit

(catalogue number LK11025 / LK0750H / LK6201H)

Please note: all protocols off site are the responsibility of the products supplier

Last modified: 2008-05-14 16:46:46