Contents > PCR & other Amplification Methods > FailSafe™ GREEN Real-Time PCR System
FailSafe™ GREEN Real-Time PCR System
Supplier: EPICENTRE® Biotechnologies
FailSafe™ GREEN Real-Time PCR Optimization Kit
| Catalogue number | Pack size |
| GFS81060 | 96 reactions |
FailSafe™ Real-Time PCR Premix-Choice Kit - Plus 2 Premixes
| Catalogue number | Pack size |
| GFS810200 | Choose any two Premixes A-L 400 reactions |
FailSafe™ Real-Time PCR Premixes - Not Available separately
| Catalogue number | Pack size |
| FSPR03A | Premix A |
| FSPR03B | Premix B |
| FSPR03C | Premix C |
| FSPR03D | Premix D |
| FSPR03E | Premix E |
| FSPR03F | Premix F |
| FSPR03G | Premix G |
| FSPR03H | Premix H |
| FSPR03I | Premix I |
| FSPR03J | Premix J |
| FSPR03K | Premix K |
| FSPR03L | Premix L |
Epicentre's® PCR products are sold under licensing arrangements with F. Hoffmann-La Roche Ltd., Roche Molecular Systems, Inc., and Applied Biosystems. The products containing a thermostable DNA polymerase are accompanied by a limited license to use it in the Polymerase Chain Reaction (PCR) and RT-PCR for life science research in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by the up-front license fee, either by payment to Applied Biosystems or as purchased, i.e., an authorised thermal cycler. For complete license statements
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Last modified: 2008-07-24 11:38:30
The FailSafe™ GREEN Real-Time PCR System uses the FailSafe™ PCR Enzyme Mix and an extensively tested set of 12 FailSafe™ GREEN PCR "PreMixes", representing various PCR conditions.
These PreMixes contain everything you need for successful quantitative PCR: SYBR®Green I dye, dNTPs, buffer, and varying amounts of MgCl2 and Epicentre's® patented FailSafe™ PCR Enhancer with Betaine.* The system also includes ROX, a passive reference fluorescent dye, which may be required for signal normalization in SYBR®Green dye reactions when using ABI real-time PCR instruments. During each round of DNA synthesis, the SYBR®Green I dye binds to the amplified PCR products and the amplicon quantity is monitored in real time by measuring its fluorescence. The incorporation of SYBR®Green I dye into a real-time PCR reaction provides great flexibility because no target-specific probes are required
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| Figure 1. Representative Performance of FailSafe GREEN Real-Time PCR System. Real-Time qPCR of the cII gene in 10-107 copies of lambda DNA using the Bio-Rad iCycler iQ™ System. Standard curve indicates a correlation coefficient of 0.999 and 100% PCR efficiency. |
Last modified: 2008-05-14 16:46:46
Applications
- Accurate, reliable real-time PCR
Benefits
- Extremely high sensitivity and specificity due to the use of a unique enzyme blend and the FailSafe™ PCR Enhancer Technology
- Successful real-time PCR the first time and every time
- Easy-to-use PreMix solution contains all components needed for quantitative PCR applications using SYBR® Green 1 dye
- No specific probes required
- Includes ROX, an internal reference dye
