Contents > Purification & Recovery of Nucleic Acids > ExtractMaster™ Fecal DNA Extraction Kit

ExtractMaster™ Fecal DNA Extraction Kit

Supplier: EPICENTRE® Biotechnologies

ExtractMaster™ Fecal DNA Extraction Kit

Catalogue number

Pack size

FD05005

5 purifications

FD05025

25 purifications

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Last modified: 2008-07-15 14:57:15

The ExtractMaster™ Fecal DNA Extraction Kit provides all of the reagents necessary to recover PCR-ready DNA from the feces of a variety of animals. The kit utilizes a detergent lysis process combined with a chromatography step, which removes enzymatic inhibitors that often co-extract with DNA from such samples. The extracted DNA is PCR-ready, and can be used with the FailSafe™ PCR System to amplify bacterial, protist or animal templates.

DNA obtained from fecal samples provides a window into animal pathophysiology. For example, changes in the methylation patterns in fecal DNA may be a promising marker for human colorectal cancer screening. In rural watersheds, E. coli typing can help track the animal sources of fecal water pollution. In addition, PCR can be used to detect intestinal protozoan infections in avian wildlife populations or in humans.

The ExtractMaster™ Fecal DNA Extraction Kit is designed to extract PCR-ready DNA in 50 minutes from a small amount (50 mg or less) of fecal matter from any of a variety of animals, including human, rat, bird, cat and cow.

Figure 1. Fecal microflora detected by PCR using primers for a eubacterial rRNA gene: 5'-CTG CTG CCT CCC GTA GGA GT and 5'-AGA GTT TGA TCC TGG CTC AG. The cycling conditions were 96° C for 3 minutes followed by 28 cycles of [96° C for 30 sec; 57° C for 30 sec; 72° C for 45 sec]. Lane 1, 100 base pair ladder; Lane 2, human fecal DNA; Lane 3, rat fecal DNA; Lane 4, rooster fecal DNA; Lane 5, no template. The amplicon is 350 bp.