Enzyme Storage Buffer
Enzyme Storage Buffer
| Catalogue number | Pack size |
Last modified: 2008-07-15 14:57:15
Enzyme Storage Buffer, for dilution of enzymes supplied in this buffer, is liquid at -20°C, but is frozen at -80°C.
Most highly-purified enzymes are stable under either of these conditions, but should not be stored at intermediate temperatures between about -30°C to -40°C because the storage buffer can freeze and thaw, potentially inactivating the enzyme. Some enzymes require storage buffers with cofactors or other components.
Confirm that Enzyme Storage Buffer is suitable for the enzyme. Some enzymes are less stable at very dilute concentrations or you may see losses in activity due to binding of the enzyme to the walls of the tube. When you dilute an enzyme, use extreme care to assure that all tubes, pipette tips, pipetters, etc. are sterile and free of nuclease contamination.
If you anticipate that you will need a different concentration of one of our enzymes on a regular basis, contact one of our Technical Consultants to ask if we could supply that concentration.
Enzyme Storage Buffer:
50% glycerol containing 50mM Tris-HCl (pH 7.5), 0.1 M NaCl, 0.1 mM EDTA, 1 mM DTT, and 0.1% Triton® X-100.
Quality Control:
Enzyme Storage Buffer is free of detectable exo- and endonuclease and RNase activities.
Last modified: 2008-05-14 16:46:46
Protocols for: Enzyme Storage Buffer
Enzyme Storage Buffer Protocol
(catalogue number ESB4901)
Please note: all protocols off site are the responsibility of the products supplier
Last modified: 2008-05-14 16:46:46
