Endonuclease IV, E. coli

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Last modified: 2008-07-15 14:57:15

Endonuclease IV, cloned from the E. coli nfo gene, is a metalloenzyme that functions in vivo to repair free radical damage in DNA. The enzyme also has Class II abasic endonuclease activity, which has utility in many areas of DNA damage and repair research. Endonuclease IV is also useful in the study of the effects of anti-tumor drugs such as bleomycin on nucleic acids in vivo.

Unit Definition:

One unit of Endonuclease IV will relax 1µg of partially depurinated, covalently closed supercoiled plasmid DNA in 30 minutes at 37°C in 50mM Tris-HCl (pH 7.5), 50mM KCl, and 1.0mM EDTA.

Storage Buffer:

50mM Tris-HCl (pH 7.5), 0.1 M NaCl, 1mM DTT, 50% glycerol, and 0.1% Triton® X-100.

Quality Control:

Endonuclease IV is tested in degradation of double-stranded DNA and is free of detectable RNase and double-stranded exonuclease activities.

Last modified: 2008-05-14 16:46:46

Protocols for: Endonuclease IV, E. coli

(catalogue number E70100)

Please note: all protocols off site are the responsibility of the products supplier

Last modified: 2008-05-14 16:46:46

References

1. Izumi, T. et al. (1992) J. Bacteriol. 174, 7711.
2. Levin, J.D. and Demple, B. (1996) Nucl. Acids Res. 24, 885.

Last modified: 2008-05-14 16:46:46