EZ-Tn5™ <DHFR-1> Insertion Kit

Catalogue number

Pack size

EZI912D

10 std reactions

EZ-Tn5™ <KAN-2> Insertion Kit

Catalogue number

Pack size

EZI982K

10 std reactions

EZ-Tn5™ <TET-1> Insertion Kit

Catalogue number

Pack size

EZI921T

10 std reactions

EZ-Tn5™ Transposase

Catalogue number

Pack size

TNP92110

10 units

Sterile Nuclease-free Water

Catalogue number

Pack size

W7350ML

50ml

TransforMax™ EC100™ Electrocompetent E. coli

Catalogue number

Pack size

EC10005

5 X 100µl

EC10010

10 X 100µl

Totally sequence cDNA or genomic clones in plasmid, cosmid, fosmid or BAC vectors without subcloning or primer walking.

EZ-Tn5™ Insertion Kits are designed to simplify and speed up complete sequencing of any cloned DNA of >2 Kb, without primer walking or subcloning. Just use a simple, one-step in vitro reaction to randomly insert a single EZ-Tn5™ Transposon containing a selectable marker - either kanamycin resistance (<KAN>), tetracycline resistance (<TET>), or trimethoprim resistance encoded by the dihydrofolate reductase gene (<DHFR>) - into the clone. Then, transform E. coli cells with an aliquot of the reaction and select for the marker encoded by the EZ-Tn5™ Transposon. Use the primers provided in the kits to sequence insertion clones bidirectionally from primer binding sites at the ends of the inserted transposon (Figure 1).

Figure 1. The process for generating DNA sequencing template using an EZ-Tn5™ Insertion Kit. Select inserts on kanamycin, tetracycline,

Last modified: 2008-05-14 16:46:46

*TransforMax™ EC100™ Electrocompetent Cells are recommended for high-efficiency, non-size-biased cloning of DNA containing EZ-Tn5™ Transposon insertions.

For Research Use Only. EZ-Tn5™ products covered by issued and pending patents

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Last modified: 2008-07-15 14:57:15

Protocols for: EZ-Tn5™ Insertion Kits

EZ-Tn5™ <DHFR-1> Insertion Kit Protocol

(catalogue number EZI912D)

EZ-Tn5™ <DHFR-1> Transposon / DHFR-1 FP-1 Forward Primer / DHRP-1 RP-1 Reverse Primer Protocol

(catalogue number DFR1001 / DFP9121 / DRP9131)

EZ-Tn5™ <KAN-2> Insertion Kit Protocol

(catalogue number EZI982K / EZI96K2)

EZ-Tn5™ <KAN-2> Transposon / KAN-2 FP-1 Forward Primer / KAN-2 RP-1 Reverse Primer Protocol

(catalogue number KAN2001 / KFP9122 / KRP0021)

EZ-Tn5™ <TET-1> Insertion Kit Protocol

(catalogue number EZI921T)

EZ-Tn5™ <TET-1> Transposon / TET-1 FP-1 Forward Primer / TET-1 RP-1 Reverse Primer Protocol

(catalogue number TET92101 / TFP9331 / TRP9341)

Please note: all protocols off site are the responsibility of the products supplier

Last modified: 2008-05-14 16:46:46

References

1. Goryshin, I.Y. and Reznikoff, W.S. (1998) J. Biol. Chem. 273, 7367.
2. Meis, R.J. (2000) EPICENTRE Forum 7(4), 5.
3. Shevchenko, Y. et al. (2002) Nucl. Acids Res. 30, 2469.
4. Shevchenko, Y. et al. (2001) EPICENTRE Forum 8(2),14.

Last modified: 2008-05-14 16:46:46