DNA Polymerase I

E. coli DNA Polymerase I catalyses three different reactions:

1. A 5'Æ3'- polymerase activity that requires a template and adds mononucleotides from deoxyribonucleoside triphosphates to the 3'-hydroxyl terminus of a primer chain.
2. A 5'Æ3'- exonuclease activity, active only on duplex DNA.
3. A 3'Æ5'- exonuclease primarily active on single-stranded DNA.

Typical preparations are greater than 95% pure, as determined by SDS polyacrylamide gel electrophoresis.

Unit Definition:

One unit is the amount of enzyme required to incorporate 10nm of total deoxyribonucleotide into acid-insoluble material in 30 min at 37°C with DNase I activated DNA as the template/primer.

Last modified: 2008-05-14 16:46:46