Centri.Spin™ 40

Centri.Spin™ 40 columns are used for the fast and efficient purification of larger molecules (proteins, nucleic acids, complex carbohydrates etc.) from small molecules (nucleotides, buffer salts etc.).
The Centri.Spin™ 40 gel will retain up to 80% of short oligonucleotides (< 25-mer) while providing excellent recovery of up to 70% of large DNA fragments with sizes greater than 135 base pairs or 20-mer, while removing > 98% of salts, NTP's and other unwanted low molecular weight impurities. Proteins, peptides, and protein conjugates >= 100 kD can also be efficiently separated/desalted from unwanted low molecular weight impurities.
Each unit consists of a specially fritted microfuge tube, dry gel, a wash tube and a sample collection tube, all specifically designed for this purpose.
The column gel is hydrated with either reagent-grade water or a suitable buffer of your choice, and spun in a microcentrifuge of swinging bucket centrifuge to remove the interstitial fluid. Your sample is then applied to the column and spun again, leaving you not only with a sample purified through the removal of low molecular weight compounds, but also exchanged into the buffer of your choice.
These columns are far superior - easy to use, quick protocol, non toxicity - than phenol/chloroform extraction, ethanol precipitation, dialysis and ultrafiltration.

Last modified: 2008-05-14 16:46:46

Protocols for: Centri.Spin™ 40

(catalogue number CS-400 / CS-401)

Please note: all protocols off site are the responsibility of the products supplier

Last modified: 2008-05-14 16:46:46