Biotin-X-X-NHS

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Last modified: 2008-07-15 14:57:15

Biotin-X-X-NHS (6-[(+)-Biotinamidocaproylamido]caproic acid N-hydroxysuccinimide ester; also referred to as Biotin-LC-LC-NHS) is a water-soluble, amine-reactive ester of biotin that can be used to label proteins or nucleic acids containing aliphatic amino (for example, aminoallyl) groups. In aqueous solution, the N-hydroxysuccinimide (NHS) group of the biotin-X-X-NHS rapidly and with high efficiency reacts with the amine group of the aminoallyl-nucleotides incorporated into RNA during an in vitro transcription reaction or into DNA during a reverse transcription or DNA-dependent DNA polymerase reaction.

Epicentre®'s Biotin-X-X-NHS is supplied as a dry powder in an air-tight vial with a self-sealing septum. The compound has been packaged under dry inert gas and is stable for at least one year when stored in powdered form in the air-tight vial at +4°C. Catalog Number BXX51005 contains five 2.5 mg vials and catalog number BXX51010 contains ten 2.5 mg vials.

Biotin-X-X-NHS is an efficient way to label aminoallyl-aRNA produced by Epicentre®'s TargetAmp™ Aminoallyl-aRNA Amplification Kits with biotin for use with Affymetrix's GeneChip® or GE Healthcare's CodeLink arrays two of the most popular microarray formats in use today.

Last modified: 2008-05-14 16:46:46

Applications

• Biotinylation of aminoallyl-aRNA (cRNA) for preparing labeled target for hybridization to microarrays.
• Biotinylation of aminoallyl-cDNA for preparing labeled target for hybridization to microarrays.
• Biotinylation of aminoallyl-RNA or aminoallyl-DNA for use as non-radioactive probe for in situ hybridization or blotting experiments.
• Biotinylation of proteins.

Benefits

• Cost-effective method for labeling RNA or DNA for microarrays, in situ hybridization and blotting experiments. Currently, labeling RNA or DNA by direct incorporation of a biotin-NTP or biotin-dNTP is very expensive due to the high cost of the biotin-(d)NTP. By incorporating an aminoallyl-(d)NTP into the DNA or RNA followed by labeling with Biotin-X-X-NHS, the user can save as much as 80% on the cost of preparing the biotin-RNA or biotin-DNA.
• Supplied as a dry powder in an air-tight vial with a self-sealing septum. The compound has been packaged under dry inert gas.
• Stable for at least one year when stored in powdered form in the air-tight vial at +4°C.
• Convenient 2.5 mg package size reduces user's cost and waste. Competitor products (Sigma, Pierce, Roche) supply Biotin-NHS is larger pack sizes (50 - 100 mg). Since only a very small amount of Biotin-NHS is needed to biotin label aminoallyl-RNA or DNA and the Biotin-NHS is unstable once it is dissolved, there is significant waste when using the competitor products.
• Protocol for labeling aminoallyl-RNA is provided with the Epicentre® Biotin-X-X-NHS. The competitors do not provide support for labeling aminoallyl-RNA.

Last modified: 2008-05-14 16:46:46

Protocols for: Biotin-X-X-NHS

(catalogue number BXX51005 / BXX51010)

Please note: all protocols off site are the responsibility of the products supplier

Last modified: 2008-05-14 16:46:46