Contents > Genomics & Proteomics > BACMAX96™ DNA Purification Kit

BACMAX96™ DNA Purification Kit

Supplier: EPICENTRE® Biotechnologies

BACMAX96™ DNA Purification Kit

Catalogue number

Pack size

BAC96066

1kit (4x96 well-plate)

BACMAX96™ DNA Purification Kit with plastic consumables

Catalogue number

Pack size

BAC96116

1 Kit

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Last modified: 2008-07-15 14:57:15

The BACMAX96™ DNA Purification Kit was developed for easy, reliable isolation of high-quality BAC and fosmid DNA in a 96-well format. The protocol is based on a modified alkaline-lysis procedure that incorporates proprietary enzyme technologies into an easy-to-use format. Purification starts with cell pellets obtained by centrifugation of 1.2-ml E.coli cultures grown in a 96-well plate. Lysates are cleared in the presence of BACMAX96™ RNase Blend which efficiently removes unwanted RNA. Following filtration and selective precipitation steps, Plasmid-Safe™ ATP-Dependent DNase is used to remove any remaining bacterial chromosomal DNA.

To maximize flexibility, culture blocks and filtration plates are supplied by the user. Filtration steps can therefore be performed via vacuum or centrifugation, making the kit easy to use with standard laboratory equipment. The kit is also amenable to semi- or fully automated purification systems.

The BACMAX96™ Kit consistently yields up to 900 ng of DNA from single-copy BAC and fosmid clones and up to 2.5 µg of DNA from CopyControl™ BAC and fosmid clones induced to high-copy number. The unprecedented yields from CopyControl clones is due in part to an autoinduction protocol that was specifically designed for a high-throughput format. In this 'hands-off' approach, Autoinduction Solution is added to the growth media prior to inoculation and copy-number remains suppressed until shortly before the cells are harvested.

Last modified: 2008-05-14 16:46:46

Application

Easy, reliable isolation of high-quality BAC and fosmid DNA in a 96-well format.

Benefits

High yields - Isolate up to 900ng of DNA from a single-copy BAC or fosmid clone and up to 2.5µg from a CopyControl BAC or fosmid clone
DNA is free of E.coli genomic DNA and RNA
'Hands-off' induction protocol designed for high-throughput formats
Easy-to-use with standard laboratory equipment
Use DNA directly for sequencing, fingerprinting, PCR and the preparation of shotgun libraries (Figure 1)

Figure 1. The BACMAX96™ DNA Purification Kit consistently yields high quality DNA that can be used directly for downstream applications like fingerprinting. Lane M, kb ladder.
A. Hind III digests of BAC DNA isolated from uninduced (-) and induced (+) CopyControl™ clones. B. Hind III digests of fosmid DNA isolated from uninduced (-) and induced (+) CopyControl clones. Digests contained one third (8µl) of the total sample volume and were analyzed by agarose gel electrophoresis.