Contents > Genomics & Proteomics > BACMAX™ DNA Purification Kit

BACMAX™ DNA Purification Kit

Supplier: EPICENTRE® Biotechnologies

BACMAX™ DNA Purification Kit

Catalogue number

Pack size

BMAX044

1 kit

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Last modified: 2008-07-15 14:57:15

The BACMAX™ DNA Purification Kit was developed for easy, reliable isolation of high-quality BAC DNA. The scalable protocol is based on a modified alkaline-lysis procedure that typically yields 0.6 to 25µg of BAC DNA from 1.5 to 100 ml of a single-copy BAC culture. Selective precipitation steps and the incorporation of EPICENTRE's® RiboShredder™ RNase Blend effectively remove contaminants that degrade DNA and interfere with downstream applications.

Last modified: 2008-05-14 16:46:46

Applications

Isolate exceptionally pure BAC DNA suitable for many applications, including sequencing, fingerprinting, PCR, and preparation of shotgun libraries.

Benefits

Higher yields of BAC DNA than with other kits (fig.1)
Consistent results (fig. 2a)
Superior quality - Isolate BAC DNA pure enough to store for one year at -20°C without being degraded (fig 2b.)
Versatile sample size - Easy to follow protocols for isolating up to 0.6, 4, 11, or 25µg of BAC DNA from 1.5, 10, 40, or 100-ml cultures of a single-copy BAC, respectively.
Convenient - Eliminate the need for columns, resins, or organic extractions.

bacmax fig 1 Obtain higher yields of BAC DNA with the BACMAX™ DNA Purification Kit

Figure 1. Obtain higher yields of BAC DNA with the BACMAX™ DNA Purification Kit. DNA was isolated from 1.5 or 3.0-ml cultures of a 153-kb single-copy BAC clone and quantitated by fluorometry with Hoechst 33258 dye. Culture volumes were those recommended by the Suppliers.

Fig 2a The BACMAX™ DNA Purification Kit consistently yields high-quality BAC DNA that can be used directly for downstream applications like fingerprinting

Panel A

Fig 2b BAC DNA isolated with the BACMAX™ Kit shows no degradation after being stored for 1 year at minus 20C

Panel B

Figure 2. Panel A. The BACMAX™ DNA Purification Kit consistently yields high-quality BAC DNA that can be used directly for downstream applications like fingerprinting. BAC DNA isolated from 10 different clones (Lanes 1 to 10) was digested with Hind III and analyzed by agarose gel electrophoresis. Lane M, kb ladder.
Panel B. BAC DNA isolated with the BACMAX™ Kit shows no degradation after being stored for 1 year at –20°C. DNA isolated from a 153-kb and a 165-kb BAC clone was immediately digested with Hind III (Lane 1, 153 kb and Lane 2, 165 kb) or stored for 1 year at -20°C and then digested with Hind III (Lane 3, 153 kb and Lane 4, 165 kb). Samples were analyzed by agarose gel electrophoresis. Lane M, kb ladder.