AmpliScribe™ T7-Flash™ Transcription Kit

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Last modified: 2008-07-15 14:57:15

The AmpliScribe™ T7-Flash™ and AmpliScribe™ T3-Flash™ Transcription Kits, Epicentre®'s new in vitro transcription kits, produce more RNA in 30 minutes than other in vitro transcription kits produce in two hours(Figure 1).
AmpliScribe™ T7-Flash™ and AmpliScribe™ T3-Flash™ Transcription reactions utilize high concentrations of NTPs that are inhibitory to conventional in vitro transcription systems. Using 1 µg of a 1.4 kb DNA template, the standard 20 µl AmpliScribe T7-Flash and AmpliScribe™ T3-Flash™ reactions produce 160 µg to 180 µg (8 to 9 mg/ml) of RNA in 30 minutes. Thus, in 30 minutes, AmpliScribe™ T7-Flash™ and AmpliScribe™ T3-Flash™ reactions produce more RNA than competitors' 'high yield' transcription kits can produce in two hours.

Double-stranded oligos, linearized plasmids or PCR products containing a phage T7 or T3 promoter are efficiently transcribed in an AmpliScribe™ T7-Flash™ or AmpliScribe™ T3-Flash™ transcription reaction.

Figure 1. AmpliScribe T7-Flash™ transcription reaction is complete in 30 minutes and yields more RNA than other 2-hour 'high-yield' transcription reactions.e Yields shown were obtained using 1µg of a linear 1.4-kb DNA template in a 20µl reaction.

Last modified: 2008-05-14 16:46:46

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Last modified: 2008-07-15 14:57:15

Protocols for: AmpliScribe™ T7 & T3-Flash™ Transcription Kits

(catalogue number ASF3057 / ASF3257 / ASF3507 / ASF03725 / ASF03750)

Please note: all protocols off site are the responsibility of the products supplier

Last modified: 2008-05-14 16:46:46