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Oligo Synthesis

Oligo Synthesis : CEPs

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2'-Se-Me-U-CE Phosphoramidite

2'-Se-Me-U-CE Phosphoramidite

Glen Research

Description

2'-Se-Me-U-CE Phosphoramidite

Structure

Catalog Number: 10-3730-xx

Description: 2'-Se-Me-U-CE Phosphoramidite

THIS ITEM HAS BEEN DISCONTINUED
5'-Dimethoxytrityl-deoxyUridine,2'-methylseleno-3'-[(2-cyanoethyl)-
(N,N-diisopropyl)]-phosphoramidite
Formula: C40H49N4O8PSe M.W.: 823.78 F.W.: 383.15
Diluent: Anhydrous Acetonitrile
Coupling: 10 minute coupling time recommended.
Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.
Storage: Refrigerated storage, maximum of 2-8°C, dry
Stability in Solution: 2-3 days

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Protocols

Glen Report 16.1

Glen Report 19.1

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Applications & Benefits

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures.

ABI 392/394
Cat.No. Pack
Size		 Grams/
Pack		 0.1M Dil.
(mL)		 LV40 LV200 40nm 0.2µm 1µm 10µm
Approximate Number of Additions
10-3730-02 0.25grams .25grams 3.03 87.67 52.6 32.88 23.91 17.53 4.38
10-3730-90 100µmoles .082grams 1 20 12 7.5 5.45 4 1
10-3730-95 50µmoles .041grams .5 3.33 2 1.25 .91 .67 .17
Expedite
Cat.No. Pack
Size		 Grams/
Pack		 Dilution
(mL)		 Molarity 50nm 0.2µm 1µm 15µm
Approximate Number of Additions
10-3730-02 0.25grams .25grams 4.53 .07 84.2 52.63 38.27 5.26
10-3730-90 100µmoles .082grams 1.5 .07 23.6 14.75 10.73 1.48
10-3730-95 50µmoles .041grams .75 .07 8.6 5.38 3.91 .54
Beckman
Cat.No. Pack
Size		 Grams/
Pack		 Dilution
(mL)		 Molarity 30nm 200nm 1000nm

Approximate Number of Additions
10-3730-02 0.25grams .25grams 4.53 .07 85.8 53.63 39

10-3730-90 100µmoles .082grams 1.5 .07 25.2 15.75 11.45

10-3730-95 50µmoles .041grams .75 .07 10.2 6.38 4.64

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