| 8-D-dG-CE Phosphoramidite |
Catalog Number: 10-1520-xx
Description: 8-D-dG-CE Phosphoramidite
5'-Dimethoxytrityl-N-dimethylformamidine-8-deutero-2'-deoxyGuanosine,
3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite |
| Formula: C43H52DN8O7P |
M.W.: 825.91 |
F.W.: 330.21 |
Diluent: Anhydrous Acetonitrile |
| Coupling: Use dmf-dG (10-1029-xx) for non-deuterated deoxyguanosine; No changes needed from standard method recommended by synthesizer manufacturer. |
| Deprotection: Use dmf-dG or UltraMild monomers. Deprotect with ammonium hydroxide for 17 hours at room temperature or deuterated ammonium hydroxide (ND4OD) for 40 hours at room temperature. Technical Bulletin |
| Storage: Refrigerated storage, maximum of 2-8°C, dry |
| Stability in Solution: 1-2 days |
Deuterated Nucleosides
Perdeuteration and selective deuteration have been useful approaches for simplification of NMR spectra and for other structural studies of large biomolecules. Driven by the progress in multinuclear multidimensional NMR spectroscopy, deuteration of nucleic acids has especially found wide applications in the NMR studies of these complex molecules in solution. 8-Deutero-2’-deoxyGuanosine phosphoramidite will be of interest to our customers involved in NMR spectroscopy.
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