Catalog Number: 26-2900-xx
Description: 3'-Phosphate PS
2-[2-(4,4'-Dimethoxytrityoxy)ethylsulfonyl]ethyl-2-succinoyl)-
polystyrene |
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F.W.: 79.98 |
Diluent: Anhydrous Acetonitrile |
| Coupling: This support should be used in a manner identical to normal protected nucleoside support since it contains the DMT group. |
| Deprotection: Cleavage of the oligonucleotide from this support requires 2 hours at room temperature with ammonium hydroxide. Complete the deprotection using the protocol required by the nucleobases. Note: complete deprotection to the 3'-phosphate requires 17 hr @ RT or 4 hr @ 55°C in ammonium hydroxide, or 8 hr @ RT in K2CO3/MeOH. Technical Bulletin |
| Storage: Freezer storage, -10 to -30°C, dry |
| Stability in Solution: 2-3 days |
STERLING SUPPORTS
All Glen Research CPG supports use the standard long chain alkylamino (lcaa) linker but differ in the glass pore size, 500Å, 1000Å or 2000Å. The 500Å support is appropriate for shorter sequences, while the 1000Å supports perform better in the synthesis of longer (>30-mer) DNA sequences. The 2000Å support is best for very long (>150-mer) oligonucleotides. We have instituted an additional QC test for supports to show the length of oligo that can be prepared before a drop-off in coupling due to steric effects begins to occur. The drop-off point is recorded in the Certificate of Analysis. All Glen Research supports are fully end-capped to ensure that the CPG surface is totally inert, thereby avoiding the introduction of impurity sequences containing deletions at the 3’-terminus.
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