Cambio - Excellence in Molecular Biology

Oligo Synthesis

Oligo Synthesis : Supports, CPGs

Prices quoted are for single packs only. For multiples of the same product please request a quote. Some of Glen's products are hazardous and may be subject to additional shipping charges. Full product information is available on Glen Research's website.

3'-dT-CPG

3'-dT-CPG

Glen Research

Catalogue No.DescriptionPack SizePriceQty
20-2084-013'-dT-CPG 0.1g £273.00 Quantity Add to Order
20-2184-413'-dT-CPG 4x1.0µm £545.00 Quantity Add to Order
20-2184-423'-dT-CPG 4x0.2µm £182.00 Quantity Add to Order

Description

3'-dT-CPG

Structure

Catalog Number: 20-2084-xx

(20-2184-xx)

Description: 3'-dT-CPG

5'-Dimethoxytrityl-3'-deoxyThymidine,2'-succinoyl-long chain alkylamino-CPG
    F.W.: 304.20

Diluent: Not Applicable
Coupling: This support should be used in a manner identical to normal protected nucleoside support since it contains the DMT group.
Deprotection: Deprotect using the protocol required by the nucleobases.
Storage: Controlled room temperature or lower, dry
Stability in Solution: Not Applicable

chain terminators

In situations where ligation must be blocked at the 5’ terminus, 5’-OMe-dT may be used. 5’-OMe modification of a strand of siRNA using 5’-OMe-T can control guide strand selection and targeting specificity.1 5’-Amino-dT terminates an oligonucleotide with a 5’-amino group which may be used for attaching a peptide or a PNA sequence. To avoid polymerase extension at the 3’ terminus, 2’,3’-dideoxynucleoside and 3’-deoxynucleoside CPGs have proved to be effective. 2’,3’- Phosphoramidites are designed to be used with the 5’-phosphoramidites and supports. Since these phosphoramidites have no DMT group, they are not compatible with purification by the DMT-on technique. Ion exchange HPLC or PAGE should be used to purify these dideoxy terminated oligos to ensure that shorter sequences (containing 3’-OH) groups are removed. (3’-Termination can also be effected using a 3’-3’ linkage formed using 5’-supports, or 3’-spacer C3 CPG.)

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

Protocols

MSDS

Glen Report

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

Applications & Benefits

To avoid polymerase extension at the 3’ terminus, 2’,3’-dideoxynucleoside and 3’-deoxynucleoside CPGs have proved to be effective.  2’,3’-phosphoramidites are designed to be used with the 5’-phosphoramidites and supports.  Since these phosphoramidites have no DMT group, they are not compatible with purification by the DMT-on technique.  Ion exchange HPLC or PAGE should be used to purify these dideoxy terminated oligos to ensure that shorter sequences (containing 3’-OH) groups are removed.  (3’-termination can also be effected using a 3’-3’ linkage formed using 5’-supports, or 3’-spacer C3 CPG.)

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

Related products

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200