Cambio - Excellence in Molecular Biology

In Vitro Toxicology

In Vitro Toxicology: Mutagenicity kits

The Ames II / Ames MPF Microfluctuation Assays are all modifications of the traditional Ames plate incorporation assay.

The Ames II assay uses the S. typhimurium strains TA98 and TAMix (a mixture of 6 strains, each detecting a specific base-pair substitution), whereas the Ames MPF® kits are available with the strains TA98, TA100, TA1535, TA1537, E.coli WP2 uvrA or E.coli WP2 pKM101.

 

Benefits of Xenometrix Ames Mutagenicity Assays over traditional Ames Procedures

  • Easy, compact and high throughput liquid format with 384 well plates.

  • Simple detection method with easy "readout" (as compared to the traditional assay)

  • Kits include all five types strains cited in OECD 471 (CoA supplied), quality controlled according to the same guideline 

  • Much less  test compound required.  (10 mg per strain if tested in triplicates, +/- S9, 6 semi-logarithmic compound dilutions)

  • Ames MPF is the preferred system for genotoxic impurities due to its low consumption of compound

  • Significantly less consumption of S9

  • Less environmental pollution due to significantly less "contaminated" waste

  • Less user intervention

  • Download presentation 

Ames MPF 100 Test Kits with semi-solid strains

The Ames MPF 100 assay comprises the histidine auxotrophic Salmonella tester strain TA100 for detecting base-pair substitutions and all media required to perform the assay. Kits including additional S9 and Positive Controls are also available.

Xenometrix AG

Description

The Ames MPF 100 assay comprises the histidine auxotrophic Salmonella tester strain TA100 for detecting base-pair substitutions and all media required to perform the assay. Kits including additional S9 and Positive Controls are also available.

Kit contents

kit includes either  1 or 10 vials  of each ( depending on the 1 sample or the 10 sample kit)

semisolid TA100
Ampicillin
Growth -, Exposure - and Reversion Indicator Media
All media and strains provided are sufficient to analyze at least 10 compounds,
triplicates, 6 concentrations, negative and positive controls, /- S9

A01-111-S1-P/A10-111-S1-P  contain the same components as the the kits but also incude  lyophilized rat liver S9; Positive Controls

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