Cambio - Excellence in Molecular Biology

PCR

PCR: Real Time PCR

Highly accurate, all-purpose RT-PCR

Thermal Cycler Validation Kits

Thermal Cycler Validation Kits for Traditional and qPCR Thermal Cyclers

Minerva Biolabs

Catalogue No.DescriptionPack SizePriceQtyAdd to Order
57-2102Traditional Thermal Cycler Validation Kit6 strips, 8 vials each £204.00£204.00Offer until : 30-Sep-2014Offer Code : MYC0809See this months offers View Offer Quantity Add to Order
57-2103Traditional Thermal Cycler Validation Kit100 Reactions £408.00£408.00Offer until : 30-Sep-2014Offer Code : MYC0809See this months offers View Offer Quantity Add to Order
57-2202qPCR Thermal Cycler Validation Kit100 Reactions £513.00£513.00Offer until : 30-Sep-2014Offer Code : MYC0809See this months offers View Offer Quantity Add to Order

Thermal Cycler Validation Kits

Thermal Cycler Validation Kits for Traditional and qPCR Thermal Cyclers

Minerva Biolabs

Type of Thermal Cycler Traditional, block heating thermal cyclers
Description False negative PCR results are highly critical and might be caused by a defective PCR cycler.  Verifcation of the correct temperature of the equipment used is essential.  The Traditional Thermal Cycler Validation Kit provides temperature-sensitive PCR reactions to monitor an upper and lower temperature range in one PCR reaction.  the primer sequences and the PCR protocol were designed to be extremely sensitive to fluctuations of >2°C within the cycler protocol with a drop out of amplification.  This way the temperature homogeneity, the precision of temperature control, and the timing can be verified.  In addition, the pre-adjusted target concentrations are only amplified at high PCR efficiences as an additional indicator for accurate temperature control of the thermal cycler/
Recommended use / Scope Applicable with any block PCR cycler in research or industrial quality assurance lab in order to fulfill legal requirements for the reliability testing of instruments used for analysis (ISO 17025, EN 45001, ISO 13485, GLP, GMP).
Kit components Lyophilised primer sets, polymerase adn nucleotides, rehydration buffer
Package size 2 verifications
Result Evaluation Gel electrophoresis at endpoint of PCR
Required consumables

PCR reaction tubes and reagents for gel electrophoresis

Required lab devices Tube centrifuge, pipetting equipment, PCR Cycler, Agarose gel electrophoresis and documentation system
Shelf life and storage Components are maintainable at 2 to 8°C for at least 6 months.  After rehydration the reagents must be stored at -18°C.

 

Type of Thermal Cycler Real-time PCR (qPCR) thermal cyclers with optical unit
Description False negative PCR results are highly critical and might be caused by a defective PCR cycler.  Verification of the correct temperature control of the equipment used is essential.  The qPCR Thermal Cycler Validation Kit provides temperature-sensitive PCR reactions to monitor an upper and lower temperature range in one PCR reaction.  The probes of each amplification system are labelled with different fluorescent dyes to allow individual evaluation.  The primer/probe sequences and the PCR protocol were designed to react extremely sensitively to incorrect temperature control, temperature homogeneity, precision and timing with a complete drop out of amplification at temperature differences of >2°C.  In addition, the pre-adjusted target concentrations are only amplified at high PCR efficiencies as an additional indicator for accurate temperature control of the thermal cycler.
Recommended use / Scope Applicable for any real-time qPCR cycler n research or industrial quality assurance lab in order to fulfill legal requirements for the reliability testing of instruments used for analysis (ISO 17025, EN 45001, ISO 13485, GLP, GMP).  The kit can be used with block as well as with air heating systems.
Kit components Lyophilised primer/probe sets, polymerase and nucleotides, rehydration buffer
Package sizes 2 verifications
Result evaluation Measuring fluorescence signal with PCR cycler
Required consumables PCR reaction tubes
Required lab devices Tube centrifuge, pipetting equipment, qPCR cycler with filters for the detection of FAM, VIC and ROX dyes.
Shelf life and storage Components are maintainable at 2 to 8°C for at least 6 months.  After rehydration the reagents must be stored at -18°C.

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