Cambio - Excellence in Molecular Biology

Cell Culture Contamination Control

Cell Culture Contamination Control: Elimination

Mycoplasma Elimination

 
If you are unfortunate enough to find mycoplasma in your cell culture or virus stocks we highly recommend immediate autoclaving and disposal of any contaminated cell cultures. After having discarded the contaminated cells along with the used reagents and cell culture media, we recommend thorough cleaning of the whole laboratory.

However, we appreciate that certain cells and virus stocks are rare or historically significant and might benefit from being cleaned up. With this purpose in mind Minerva Biolabs have developed mycoplasma elimination products (Mynox® and Mynox® Gold) that can eradicate mycoplasma from an already contaminated cell culture with negligible cytotoxicity.

Mynox® is an antibiotic-free formulation for mycoplasma eradication from cell cultures. Its activity is based on a biophysical mechanism and therefore does not allow for the development of resistant strains.


Mynox® Gold is a formulation of the classic Mynox® reagent that has undergone further development and combines a standard antibiotic with the Mynox® ingredients. Achieve 100% eradication of mycoplasma with no cytotoxicity and a low resistance risk.

MycoRAZOR™

the MycoRAZOR™ Mycoplasma elimination kit

Biontex

Catalogue No.DescriptionPack SizePriceQty
M040-100MycoRAZOR™1 £134.00 Quantity Add to Order

Description

 

Cell cultures must be screened periodically for possible mycoplasmal contamination. The use of different methods is recommended to ensure detection (e.g. MycoSCOUT™). Detection is necessary when working with both established cell lines and primary material. 

If mycoplasmas have been detected in your cell culture, the highly effective mixture of antibiotics MycoRAZOR® eliminates the contamination rapidly and efficiently.

It acts on the protein biosynthesis mechanism by interfering with the ribosome translation of the mycoplasmas as well as with their transcription (gyrase blocker) without impacting on the eukaryotic cells in the culture.

Treatment of cell cultures is easy: simply add MycoRAZOR® to your culture medium to kill mycoplasmas and bacteria in the culture rapidly and completely. 

Recommended final concentration: 20 ml/l

Product Specifications
pH 4.0 – 4.5 Sterility tested
Solvent PBS Cell Culture tested
Osmolality 280 – 340 mOsmol/kg Shelf Life 2 Years
Endotoxin < 10 EU/ml Storage < -15°C

 

Application Removes mycoplasmas from contaminated cell cultures
Formulation Antibiotic mixture in PBS
Assays Approx. 300 applications (T75) per 100 ml reagent
Recommended final
concentration
20 μl/ml
pH 4.0 – 4.5
Osmolality 280 – 340 mOsmol/kg
Endotoxin < 10 EU/ml
Sterility Tested
Shipping In dry ice
Storage ≤ –15°C
Shelf Life 2 years (after date of delivery)

Biontex Mycorazor

 




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If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

Applications & Benefits

Application

  • Removes mycoplasmas from contaminated cell cultures

Benefits

    • Easy Protocol
    • Guaranteed mycoplasma removal
    • Effective against all mycoplasma
    • No effect on eukaryotic cell proliferation
    • Cost-effective

 

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

Technical Help

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Related products

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200