Cambio - Excellence in Molecular Biology

Transfection-Biontex

Transfection-Biontex: Proteofection

Transfection of proteins into mammalian cells

Micro-Proteofection Kit VI

Cell culture, proteofection and high-resolution microscopy directly in the culture vessel

Biontex

Catalogue No.DescriptionPack SizePriceQty
K030-0.25 Micro-Proteofection Kit VI250ul £1,037.00 Quantity Add to Order

Description

Like the μ-Transfection Kit VI (cat. no. K010), μ-Proteofection Kit VI, developed especially for proteofection, is a unique combination of μ-Slides VI from ibidi and PROTEOfectene™ from Biontex. The high-performance kit enables living proteofected cells to be observed under high-resolution microscopy.

In the ultra-simple protocol, adherent cells are treated in the μ-Slide with proteoplexes formed from PROTEOfectene™ and a corresponding protein, and can subsequently be observed directly in excellent optical quality. Examples of applications include tracking the progress of a fluorescence-tagged protein through the cell from endocytosis to its place of action.

  • Immediate live observation possible
  • Ideal optical properties for transmitted light and fluorescence microscopy
  • Optimum cell vitality
  • Full serum compatibility
  • Visualization of transfection process in living cells
  • Efficient, rapid protocol
  • Cells can be fixed and dyed
  • Compatible with oil immersion

 

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References

E. Conti, J. Kuriyan; Structure Fold. Des. v8, 329 – 338 (2000) [PubMed ID: 10745017]

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Notes

  
   
   
   
   
   
   

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Applications & Benefits

Application Proteofection and high-resolution microscopy of living eukaryotic cells (live cell imaging)
Content μ-Slides VI (6 channels each), PROTEOfectene™, R-Phycoerythrin as positive control
Assays 90 per kit
Shipping At room temperature
Storage 4°C
Shelf Life 1 year (after date of delivery)

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