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Oligo Synthesis

Oligo Synthesis : CEPs

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Long Trebler Phosphoramidite

Long Trebler Phosphoramidite

Glen Research

Catalogue No.DescriptionPack SizePriceQty
10-1925-02Long Trebler Phosphoramidite 0.25g £273.00 Quantity Add to Order
10-1925-90Long Trebler Phosphoramidite 100µmoles £182.00 Quantity Add to Order

Description

Long Trebler Phosphoramidite

Structure

Catalog Number: 10-1925-xx

Description: Long Trebler Phosphoramidite

Tris-2,2,2-[3-(4,4'-dimethoxytrityloxy)propyloxymethyl]methyleneoxypropyl-
[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
Formula: C89H107N2O15P M.W.: 1475.78 F.W.: 428.41
Diluent: Anhydrous Acetonitrile
Add fresh diluent to product vial to recommended concentration and swirl vial occasionally over several minutes until product is completely dissolved. (Some oils may require between 5 and 10 minutes.) Use care to maintain anhydrous conditions. In case of transfer to alternate vial type, ensure recipient vial has been pre-dried. For more information, see http://www.glenres.com/ProductFiles/Technical/TB_ABITransfer.pdf.
Coupling: Synthesis should be carried out on 1000 support. A 15 minute coupling time using DCI activator (30-3150-xx) is recommended for the trebler addition.
Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.
Storage: Freezer storage, -10 to -30°C, dry
Stability in Solution: 1-2 days

Dendrimers

Dendrimers are discrete, highly branched, monodispersed polymers that possess patterns reminiscent of the branching of trees. Plain and mixed oligonucleotide dendrimers can be synthesized using novel doubling and trebling phosphoramidite synthons.1,2 Dendrimers offer the following advantages. Incorporation of label using γ-32P-ATP and polynucleotide kinase increases in proportion to the number of 5’-ends. Fluorescent signal also increases in proportion to the number of 5’-ends, if spacers are incorporated between the labels and the ends of the branches. When using a dendrimeric oligonucleotide as a PCR primer, the strand bearing the dendrimer is resistant to degradation by T7 Gene 6 exonuclease making it easy to convert the double-stranded product of the PCR to a multiply labelled, single-stranded probe. Enhanced stability of DNA dendrimers makes them useful as building blocks for the ‘bottom up’ approach to nano-assembly. These features also suggest applications in DNA chip technology when higher temperatures are required, for example, to melt secondary structure in the target.

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Protocols

Material Safety Data Sheet

Glen Report 19.1: Microarrays, Nanotechnology and Beyond

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References

(1) M.S. Shchepinov, I.A. Udalova, A.J. Bridgman, and E.M. Southern, Nucleic Acids Res, 1997, 25, 4447-4454.

(2) M.S. Shchepinov, K.U. Mir, J.K. Elder, M.D. Frank-Kamenetskii, and E.M. Southern, Nucleic Acids Res, 1999, 27, 3035-41.

(3) T. Horn, C.A. Chang, and M.S. Urdea, Nucleic Acids Res, 1997, 25, 4842-4849.

 

Doublers and Trebler are supplied under license from ISIS Innovation Limited.

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Notes

Long Trebler Phosphoramidite

Tris-2,2,2-[3-(4,4'-dimethoxytrityloxy)propyloxymethyl]methyleneoxypropyl-
[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
Formula: C89H107N2O15P M.W.: 1475.78 F.W.: 428.41
Diluent: Anhydrous Acetonitrile
Coupling: Synthesis should be carried out on 1000 support. A 15 minute coupling time using DCI activator (30-3150-xx) is recommended for the trebler addition.
Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.
Storage: Freezer storage, -10 to -30°C, dry
Stability in Solution: 1-2 days
  Material Safety Data Sheet

Literature Highlights

Glen Report 19.1: Microarrays, Nanotechnology and Beyond

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.

ABI 392/394
Cat.No. Pack
Size		 Grams/
Pack		 0.1M Dil.
(mL)		 LV40 LV200 40nm 0.2µm 1µm 10µm
Approximate Number of Additions
10-1925-90 100µmoles .148grams 1 20 12 7.5 5.45 4 1
10-1925-02 0.25grams .25grams 1.69 43 25.8 16.13 11.73 8.6 2.15
Expedite
Cat.No. Pack
Size		 Grams/
Pack		 Dilution
(mL)		 Molarity 50nm 0.2µm 1µm 15µm  
Approximate Number of Additions
10-1925-90 100µmoles .148grams 1.5 .067 23.6 14.75 10.73 1.48  
10-1925-02 0.25grams .25grams 2.53 .067 44.2 27.63 20.09 2.76  
Beckman
Cat.No. Pack
Size		 Grams/
Pack		 Dilution
(mL)		 Molarity 30nm 200nm 1000nm    
Approximate Number of Additions
10-1925-90 100µmoles .148grams 1.5 .067 25.2 15.75 11.45    
10-1925-02 0.25grams .25grams 2.53 .067 45.8 28.63 20.82

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Applications & Benefits

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.

ABI 392/394
Cat.No. Pack
Size		 Grams/
Pack		 0.1M Dil.
(mL)		 LV40 LV200 40nm 0.2µm 1µm 10µm
Approximate Number of Additions
10-1925-90 100µmoles .148grams 1 20 12 7.5 5.45 4 1
10-1925-02 0.25grams .25grams 1.69 43 25.8 16.13 11.73 8.6 2.15
Expedite
Cat.No. Pack
Size		 Grams/
Pack		 Dilution
(mL)		 Molarity 50nm 0.2µm 1µm 15µm
Approximate Number of Additions
10-1925-90 100µmoles .148grams 1.5 .07 23.6 14.75 10.73 1.48
10-1925-02 0.25grams .25grams 2.53 .07 44.2 27.63 20.09 2.76
Beckman
Cat.No. Pack
Size		 Grams/
Pack		 Dilution
(mL)		 Molarity 30nm 200nm 1000nm

Approximate Number of Additions
10-1925-90 100µmoles .148grams 1.5 .07 25.2 15.75 11.45

10-1925-02 0.25grams .25grams 2.53 .07 45.8 28.63 20.82

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

Related products

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