Cambio - Excellence in Molecular Biology

Oligo Synthesis

Oligo Synthesis : CEPs

Prices quoted are for single packs only. For multiples of the same product please request a quote. Some of Glen's products are hazardous and may be subject to additional shipping charges. Full product information is available on Glen Research's website.

Epoch Gig Harbor GreenTM Phosphoramidite

Epoch Gig Harbor GreenTM Phosphoramidite

Glen Research

Description

Epoch Gig Harbor GreenTM Phosphoramidite

Structure

Catalog Number: 10-5922-xx

Description: Epoch Gig Harbor Green™Phosphoramidite

3-(3',6'-dipivaloylfluorescein-2-yl)-pentanamidohexyl-
1-O-(2-cyanoethyl)-(N,N-diisopropyl)-phosphoramidite
Formula: C50H66N3O10P M.W.: 900.06 F.W.: 594.57
Diluent: Anhydrous Acetonitrile
Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.
Storage: Freezer Storage, -10 to -30°C, dry
Stability in Solution: 2-3 days
Please Note: This Product is for research purposes only, and may not be used for commercial, clinical, diagnostic or any other use. The Product is subject to proprietary rights of Epoch Biosciences, Inc. and is made and sold under license from Epoch Biosciences, Inc. There is no implied license for commercial use with respect to this Product and a license must be obtained directly from Epoch Biosciences, Inc. with respect to any proposed commercial use of this Product. "Commercial use" includes but is not limited to the sale, lease, license or other transfer of the Product or any material derived or produced from it, the sale, lease, license or other grant of rights to use the Product or any material derived or produced from it, or the use of the Product to perform services for a fee for third parties (including contract research). Eclipse, Gig Harbor Green, Redmond Red and Yakima Yellow are trademarks of Epoch Biosciences, Inc.

Please see Terms and Conditions of Use

EPOCH DYES AND QUENCHER

Glen Research’s agreement with Epoch Biosciences, Inc., a subsidiary of Nanogen, Inc., allows us to sell several of Epoch’s proprietary products designed for the synthesis of novel DNA probes. We are pleased to offer products based on Epoch’s Redmond Red™, Yakima Yellow™ and Gig Harbor Green™ fluorophores and Eclipse™ non-fluorescent quencher. Under our agreement we also supply PPG, a modified nucleoside and 5’-Aldehyde-Modifier C2 Phosphoramidite. The two fluorescent dyes, Yakima Yellow and Redmond Red, are available as phosphoramidites and supports. Yakima Yellow has an absorbance maximum at 530 nm and emission maximum at 549 nm, while Redmond Red’s absorbance and emission maxima are at 579 nm and 595 nm, respectively. Gig Harbor Green and 6-FAM are based on the same fluorescein core structure but Gig Harbor Green is 15-20% brighter than FAM.

The Eclipse quencher from Epoch solves most of the problems inherent in the synthesis of molecular beacon and FRET probes. The Eclipse molecule is highly stable and can be used safely in all common oligo deprotection schemes. The absorbance maximum for Eclipse Quencher is at 522 nm, compared to 479 nm for dabcyl. In addition, the structure of the Eclipse Quencher is substantially more electron deficient than that of dabcyl and this leads to better quenching over a wider range of dyes, especially those with emission maxima at longer wavelengths (red shifted) such as Redmond Red and Cy5. In addition, with an absorption range from 390 nm to 625 nm, the Eclipse Quencher is capable of effective performance in a wide range of colored FRET probes.

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

Protocols

Material Safety Data Sheet

Glen Report 16.2: NEW EPOCH PRODUCTS - 5'-Aldehyde-Modifier C2 phosphoramidite and gig harbor green™ phosphoramidite

 

home


CATALOG PRODUCTS ARCHIVES FEEDBACK SEARCH

  alphbetical index Catalog Number index MSDS index Deprotection Volumes 1-5 User guide to Purification Literature Highlights Listing of oligo houses FAQ  

Catalog Number: 10-5922-xx

Description: Epoch Gig Harbor Green™Phosphoramidite

3-(3',6'-dipivaloylfluorescein-2-yl)-pentanamidohexyl-
1-O-(2-cyanoethyl)-(N,N-diisopropyl)-phosphoramidite
Formula: C50H66N3O10P M.W.: 900.06 F.W.: 594.57
Diluent: Anhydrous Acetonitrile
Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.
Storage: Freezer Storage, -10 to -30°C, dry
Stability in Solution: 2-3 days
Please Note: This Product is for research purposes only, and may not be used for commercial, clinical, diagnostic or any other use. The Product is subject to proprietary rights of Epoch Biosciences, Inc. and is made and sold under license from Epoch Biosciences, Inc. There is no implied license for commercial use with respect to this Product and a license must be obtained directly from Epoch Biosciences, Inc. with respect to any proposed commercial use of this Product. "Commercial use" includes but is not limited to the sale, lease, license or other transfer of the Product or any material derived or produced from it, the sale, lease, license or other grant of rights to use the Product or any material derived or produced from it, or the use of the Product to perform services for a fee for third parties (including contract research). Eclipse, Gig Harbor Green, Redmond Red and Yakima Yellow are trademarks of Epoch Biosciences, Inc.
Please see Terms and Conditions of Use
Catalog Information Material Safety Data Sheet

LITERATURE HIGHLIGHTS

Glen Report 16.2: NEW EPOCH PRODUCTS - 5'-Aldehyde-Modifier C2 phosphoramidite and gig harbor green™ phosphoramidite

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

Applications & Benefits

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.

ABI 392/394
Cat.No. Pack
Size		 Grams/
Pack		 0.1M Dil.
(mL)		 LV40 LV200 40nm 0.2µm 1µm 10µm
Approximate Number of Additions
10-5922-02 0.25grams .25grams 2.78 79.33 47.6 29.75 21.64 15.87 3.97
10-5922-90 100µmoles .09grams 1 20 12 7.5 5.45 4 1
10-5922-95 50µmoles .045grams .5 3.33 2 1.25 .91 .67 .17
Expedite
Cat.No. Pack
Size		 Grams/
Pack		 Dilution
(mL)		 Molarity 50nm 0.2µm 1µm 15µm
Approximate Number of Additions
10-5922-02 0.25grams .25grams 4.15 .07 76.6 47.88 34.82 4.79
10-5922-90 100µmoles .09grams 1.5 .07 23.6 14.75 10.73 1.48
10-5922-95 50µmoles .045grams .75 .07 8.6 5.38 3.91 .54
Beckman
Cat.No. Pack
Size		 Grams/
Pack		 Dilution
(mL)		 Molarity 30nm 200nm 1000nm

Approximate Number of Additions
10-5922-02 0.25grams .25grams 4.15 .07 78.2 48.88 35.55

10-5922-90 100µmoles .09grams 1.5 .07 25.2 15.75 11.45

10-5922-95 50µmoles .045grams .75 .07 10.2 6.38 4.64

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

Related products

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200