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Oligo Synthesis

Oligo Synthesis : CEPs

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dG-H-Phosphonate, TEA Salt

dG-H-Phosphonate, TEA Salt

Glen Research

Description

dG-H-Phosphonate, TEA Salt

Structure

Catalog Number: 10-1220-xx

Description: dG-H-Phosphonate, TEA Salt

5'-Dimethoxytrityl-N-isobutyryl-2'-deoxyGuanosine,
3'-H-phosphonate, TEA salt
Formula: C41H53N6O9P M.W.: 804.88 F.W.: 329.21
Diluent: Acetonitrile/Pyridine (1:1)
Coupling: H-Phosphonate Synthesis Protocol
Deprotection: No changes needed from standard method recommended by synthesizer manufacturer. Technical Bulletin
Storage: Refrigerated storage, maximum of 2-8°C, dry
Stability in Solution: ~1week

h-phosphonate monomers

Glen Research H-Phosphonates are analyzed by HPLC and are synthesis-tested. H-Phosphonates are especially useful for the preparation of modified internucleotide linkages which are unattainable by phosphoramidite chemistry. The most popular application is the preparation of radiolabelled phosphorothioates, since the sulfurization reaction is carried out off the synthesizer. These monomers are packaged in ABI-style vials.

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Protocols

Material Safety Data Sheet

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Notes

Frequently Asked Technical Question

QUESTION: What are the concentrations of the solutions used on an ABI for H-phosphonate chemistry?

RESPONSE:1. Adamantantanecarbonyl chloride - 2% w/v in 5%pyridine/acetonitrile.

2. Isopropyl phosphite - 1% in acetonitrile/pyridine (50:50)

3. Oxidation: Following synthesis

Step 1: React support with 4% Iodine in THF/Pyridine/Water ( 8:1:1) ~2mL for 4minutes

Step 2: Remove solution from support

Step 3: React support with mixture of 4% Iodine in THF/Pyridine/Water ( 8:1:1) (~1mL) and TEA/H2O/THF (8:1:1) (~1mL) for 3 minutes

Step 4: Flush column with acetonitrile and blow dry support

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Applications & Benefits

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.

ABI 392/394
Cat.No. Pack
Size		 Grams/
Pack		 0.1M Dil.
(mL)		 LV40 LV200 40nm 0.2µm 1µm 10µm
Approximate Number of Additions
10-1220-02 0.25grams .25grams 21 686.67 412 257.5 187.27 137.33 34.33
10-1220-05 0.5grams .5grams 42 1386.67 832 520 378.18 277.33 69.33
Expedite
Cat.No. Pack
Size		 Grams/
Pack		 Dilution
(mL)		 Molarity 50nm 0.2µm 1µm 15µm
Approximate Number of Additions
10-1220-02 0.25grams .25grams 5 .06 93.6 58.5 42.55 5.85
10-1220-05 0.5grams .5grams 10 .06 193.6 121 88 12.1
Beckman
Cat.No. Pack
Size		 Grams/
Pack		 Dilution
(mL)		 Molarity 30nm 200nm 1000nm

Approximate Number of Additions
10-1220-02 0.25grams .25grams N/A N/A N/A N/A
10-1220-05 0.5grams .5grams N/A N/A N/A N/A

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Related products

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