Enzymes for Molecular Biology
The range of applications for the enzymes in this section is extensive. Polymerases are useful for labeled DNA probes by nick translation, second-strand cDNA synthesis, strand-displacement amplification and PR and multiplex PCR amplification. Ligases can be applied to ligation of blunt or cohesive-ended DNA fragments, ligation amplification (LCR), repeat Expansion Detection (RED) and molecular cloning of single stranded and double stranded DNA. Other molecular cloning enzymes listed are useful for DNA repair research and study of UV damaged DNA.
CviJ I is the only available restriction enzyme recognising two/three basepair sequence, thus allowing for new research applications. This version of CviJ I cleaves only PuGCPy sites. It does not exhibit ‘star’ activity, thus it is better suited for mapping of short DNAs like PCR products or small plasmids. Other CviJ I applications, like shotgun cloning, thermal cycle labelling or epitope mapping, can be performed either with this version or with CviJ I*, also available from Cambio.