Active, purified GLP1R (Glucagon-like peptide 1 receptor)
Membrane proteins are the most pharmaceutically relevant protein class. At the same time, it is very difficult to obtain them in pure, active form. Cube Biotech's protein experts have worked hard to produce human GPCRs in sufficient quality that we can now offer to the community.
We offer active, purified GLP1R, a human GPCR involved in insuline production and appetite regulation.
The protein is available off-the shelf, ready for ligand binding studies, crystallization, and other biochemical/biophysical experiments.
Protein
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GLP1R (Glucagon-like peptide 1 receptor)
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Alternative names
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GLP1-receptor |
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UniProt number
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P43220
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Protein class
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GPCR, class B |
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Organism
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Human (Homo sapiens) |
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Sequence
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Full-length, wildtype sequence
N-terminal HA tag (underlined), initial methionine in bold, C-terminal 10x His-tag in red,
HRV 3C protease site in blue, spacer in bold grey, Rho1D4 tag in bold green
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MKTIIALSYIFCLVFARPQGATVSLWETVQKWREYRRQCQRSLTEDPPPATDLFCNRTFDEYACWPDGEPGSFVNVSCP
WYLPWASSVPQGHVYRFCTAEGLWLQKDNSSLPWRDLSECEESKRGERSSPEEQLLFLYIIYTVGYALSFSALVIASAIL
LGFRHLHCTRNYIHLNLFASFILRALSVFIKDAALKWMYSTAAQQHQWDGLLSYQDSLSCRLVFLLMQYCVAANYYWLLV
EGVYLYTLLAFSVLSEQWIFRLYVSIGWGVPLLFVVPWGIVKYLYEDEGCWTRNSNMNYWLIIRLPILFAIGVNFLIFVRVI
CIVVSKLKANLMCKTDIKCRLAKSTLTLIPLLGTHEVIFAFVMDEHARGTLRFIKLFTELSFTSFQGLMVAILYCFVNNEVQL
EFRKSWERWRLEHLHIQRDSSMKPLKCPTSSLSSGATAGSSMYTATCQASCSGGHHHHHHHHHHLEVLFQGPGSSG
TETSQVAPA
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Affinity tags
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His / Rho1D4 (both C-terminal) |
Size (excluding
additional elements)
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489 (463) amino acids
56,170 (53,026) Da
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Expression system
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Sf9 (baculovirus) |
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Purified via
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Protein-specific affinity matrix using immobilized agonist ligand |
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Buffer
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20 mM HEPES pH 7.5, 150 mM NaCl, 10% Glycerol, 0.02% Foscholine-12 |
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Purity (SDS-PAGE)
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>98% as determined by SDS-PAGE |
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Homogeneity
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Size exclusion chromatography |
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Activity
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Ligand binding measured by SPR using the agonist ligand exendin-4 at 10°C. Using a 1:1 binding model, the kD was determined to be 6.11 x 10e7 M, which is in accordance with published values.
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Function
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Receptor for glucagon-like peptide 1. Expressed in pancreas, brain, heart, kidney and the GI tract. Involved in insuline secretion and appetite regulation.
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Literature references:
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1. Hwang JI et al. Molecular evolution of GPCRs: GLP1/GLP1 receptors. J. Mol. Endocrinol. (2014) 52 (3) 15-27.
2. Sisley S. et al. Neuronal GLP1R mediates liraglutide's anorectic but not glucose-lowering effect. (2014) J. Clin. inv. 124(6)2456-2463.
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If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200