Diluent: Anhydrous Acetonitrile
Coupling: 3 minute coupling time recommended.
Deprotection: No changes needed from standard method recommended by synthesizer manufacturer. NOTE: a side-reaction can occur post-synthesis that leads to cleavage of phosphodiester linkages vicinal to the COT. To avoid this side-reaction, deprotect the oligo soon after the oligonucleotide synthesis is completed. Otherwise, store the column at -20°C until the deprotection can be initiated. Avoid drying down the oligo with heat.
Storage: Storage: Freezer storage, -10 to -30°C, dry
Stability in Solution: 1-2 days
Please Note: This product is covered by US Patent Nos.: 8,394,948 and 8,945,515 B2.
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