Description
The GetClone™ PCR Cloning Vector is a positive selection system for the high efficiency cloning of blunt end DNA or amplicons. This cloning vector contains a lethal gene which can be disrupted by ligation with a blunt end DNA insert at the cloning site. Only colonies with inserted vectors are able to propagate, eliminating the additional needs of IPTG and X-Gal for blue/ white screening.
Features
• Cloning efficiency was greater than 90%
• The IPTG and X-Gal are not required
• Accepts a wide range of insert/ vector ratio 0.5:1 to 12:1
• Accepts insert size from 6 bp to 12 kb
• The phosphorylation of PCR fragments is not required
• Accepts blunt end amplicon or DNA fragment (not for sticky end)
Contents
Component
Volume
| pGet1.1 vector |
23 μl |
| pGet-For primer (10 µM) |
100 μl |
| pGet-Rev primer (10 µM) |
100 µl |
Storage -20°C ≥ 24 months
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