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Oligo Synthesis

Oligo Synthesis : CEPs

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tC-CE Phosphoramidite

tC-CE Phosphoramidite

Glen Research

Description

STructure

 

 

Catalog Number: 10-1516-xx

Description: tC-CE Phosphoramidite

5'-O-(4,4'-Dimethoxytrityl)-1'-(1,3-diaza-2-oxophenothiazin-1-yl)-
2'-deoxy-Β-D-ribofuranosyl-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
Formula: C45H50N5O7PS M.W.: 835.95 F.W.: 395.33
Diluent: Anhydrous Acetonitrile
Couping: 3 minute coupling time.
Deprotection: As required by nucleobases. Compatible with Ammonium Hydroxide for 17 hours at room temperature, Ammonium hydroxide/40% methylamine 1:1 (AMA) for 10 minutes at 65°C or UltraMild deprotection conditions.
Storage: Freezer storage, -10 to -30°C, dry
Stability in Solution: 1-2 days

Please Note: These products are offered under license from ISIS Pharmaceuticals, Inc. and in collaboration with ModyBase HB.

The tricyclic fluorescent nucleoside analogues, 1,3-diaza-2-oxophenothiazine, tC, and 1,3-diaza-2-oxophenoxazine, tCo, are deoxycytidine analogs that have been shown to base pair faithfully with dG with virtually no disruption of the normal duplex structure. This means that the stability of the DNA duplex is not compromised as compared to the control regardless of DNA sequence. The fluorescence quantum yield of tC is essentially unchanged between single stranded and double stranded DNA - 0.21 for single stranded DNA and 0.19 for duplex DNA. Also, the fluorescence characteristics of tC are not sensitive to neighboring base combinations. tCo has been shown to be the brightest fluorescent nucleoside analogue in duplex context reported so far and even retains the majority of its fluorescence when surrounded by guanine residues. Indeed, tCo has been reported to be 25-50 times brighter than 2-aminopurine.









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Notes

Please Note: These products are offered under license from ISIS Pharmaceuticals, Inc. and in collaboration with ModyBase HB.

Absorption and emisssion data for tC and tCo are collected below:

 

tC
QY
l
e
(L/mol.cm)
single-stranded
0.21
385nm
 
double-stranded
0.19
 
 
tCo
QY
l
e
(L/mol.cm)
single-stranded
0.30
360nm
9000
double-stranded
0.21

(QY determined relative to quinine sulfate in 0.5M H2SO4)

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Applications & Benefits

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.

ABI 392/394
Cat.No. Pack
Size		 Grams/
Pack		 0.1M Dil.
(mL)		 LV40 LV200 40nm 0.2µm 1µm 10µm
Approximate Number of Additions
10-1516-95 50µmoles .042grams .5 3.33 2 1.25 .91 .67 .17
10-1516-90 100µmoles .084grams 1 20 12 7.5 5.45 4 1
10-1516-02 0.25grams .25grams 2.99 86.33 51.8 32.38 23.55 17.27 4.32
Expedite
Cat.No. Pack
Size		 Grams/
Pack		 Dilution
(mL)		 Molarity 50nm 0.2µm 1µm 15µm
Approximate Number of Additions
10-1516-95 50µmoles .042grams .75 .07 8.6 5.38 3.91 .54
10-1516-90 100µmoles .084grams 1.5 .07 23.6 14.75 10.73 1.48
10-1516-02 0.25grams .25grams 4.46 .07 82.8 51.75 37.64 5.18
Beckman
Cat.No. Pack
Size		 Grams/
Pack		 Dilution
(mL)		 Molarity 30nm 200nm 1000nm

Approximate Number of Additions
10-1516-95 50µmoles .042grams .75 .07 10.2 6.38 4.64

10-1516-90 100µmoles .084grams 1.5 .07 25.2 15.75 11.45

10-1516-02 0.25grams .25grams 4.46 .07 84.4 52.75 38.36

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Related products

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